FastQCFastQC Report
Tue 17 Aug 2021
HGFHNDRXY_n01_col1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFHNDRXY_n01_col1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140005591
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGACTTATCTCGTA4934470.35244806759181496TruSeq Adapter, Index 25 (97% over 38bp)
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4714440.33673226664212286No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGACTTATCGCGTA2596290.18544187996035102TruSeq Adapter, Index 25 (97% over 38bp)
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2517030.17978067747308749No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1860240.13286897949668308No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)