FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_untreated_200_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_untreated_200_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13035810
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAT44350.042.77221335
ACTCCCG48750.039.55727432
CACGTCT53600.036.9575114
AGTCACT58050.033.94328728
ACACGTC59300.033.7003313
GTCACTC58500.033.50283429
CACACGT60200.033.4290512
ACGTCTG59550.033.3236315
CGTCTGA62700.031.7051816
CAGTCAC66950.030.21516427
CCGAATC58850.028.90319436
TCACTCC70900.028.08765230
GTCTGAA72550.027.6899517
ATCGGAA73300.027.1734182
TCGGAAG74100.027.0688063
CACTCCC73800.026.93651231
CGAATCT35400.026.39777837
CTCCCGA74800.025.92134733
GCACACG81300.025.09751511
GATCGGA81750.024.5384921
Produced by FastQC (version 0.11.2)