FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_untreated_200_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_untreated_200_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8611732
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGC468390.5438975574251498TruSeq Adapter, Index 3 (97% over 36bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT105980.12306467502704452No Hit
CGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCG88130.10233713729131376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG57350.065.853646
GTATGCC59100.064.13978645
TCGTATG59400.063.6972543
CTCGTAT53400.063.24991242
CACGTCT65450.062.51021614
ACACGTC66550.061.47770313
ACGTCTG67600.060.3667815
ATGCCGT62750.060.35386747
TCACTCA66050.059.8758630
GCCGTCT64650.058.30941449
CGTATGC65800.058.14076644
TGCCGTC66250.057.16535648
CTGAACT72050.056.54088619
GAACTCC72500.055.90030721
GAGCACA74250.055.243979
TCATTCA73000.053.31361834
ACTCATT74050.053.31294332
AGTCACT75400.053.28643828
GTCACTC74450.053.2145529
CACTCAT74950.052.85954331
Produced by FastQC (version 0.11.2)