FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_untreated_025_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_untreated_025_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12530681
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTATGC301100.24029021247927387TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC46150.048.0840144
TATGCCG49700.044.79082546
TATCTCG49650.043.7762139
CTATAAT54150.042.592933
TCGTATG53450.041.9091143
GTATGCC53300.041.43671445
CACTATA57050.040.42778831
CTCGTAT54400.039.76098642
ACTATAA60400.038.99674632
AGTCACT59100.038.96625528
CACGTCT63300.036.8234314
ACGTCTG63350.036.7391215
TAATATC60400.036.38981636
GTCACTA64400.035.8138929
TATAATA64600.035.81136734
CACACGT66150.035.6604812
ACACGTC66250.035.23671313
CGTCTGA66250.035.13077516
CAGTCAC67900.034.99870327
ATGCCGT68600.032.2974347
Produced by FastQC (version 0.11.2)