FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_untreated_025_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_untreated_025_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9345595
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT27850.033.5537214
ACGTCTG28250.033.45029415
ACACGTC30600.030.53843713
AGTCACT31200.029.05296728
CACTACA32100.027.36617331
ACTACAG32800.027.20894232
CAGTCAC39050.024.64672927
CACACGT39950.023.3912712
CGTCTGA41600.022.71564716
GTCTGAA43650.021.88912417
GTCACTA41550.021.14209629
CTGAACT44400.020.96737319
TCTGAAC48500.019.70021218
TCACTAC45500.019.61435930
TCGGAAG47500.019.455053
TATGCCG11250.018.97881746
AGCACAC51250.018.9165910
TCCAGTC48150.018.75301425
ATCGGAA50900.018.2244642
GATCGGA51550.018.200331
Produced by FastQC (version 0.11.2)