FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_5000_200_r.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_5000_200_r.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12300772
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACATCTCGTATGC218520.17764738668434793TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG39250.048.33282546
CGTATGC42300.045.4263144
GTATGCC45200.042.04718445
TCGTATG47600.040.4418743
CACGTCT51600.039.54348414
CTCGTAT46650.039.2388942
CTATACA51900.038.50552734
ACCTATA53900.036.8167232
CACCTAT54050.036.45553631
ACACGTC57050.035.8887313
CAGTCAC57000.035.1214127
AGTCACC57150.035.02923228
ACGTCTG58550.034.9690115
CCTATAC58950.033.7222933
CACACGT61600.033.1813112
ATGCCGT59600.032.652147
TATACAT62700.031.87312535
TCACCTA63100.031.22696930
TCTCGTA61200.030.31036241
GTCTGAA67900.030.05047417
Produced by FastQC (version 0.11.2)