FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_5000_200_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_5000_200_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8685025
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGATCCAATCTCGTAT160560.18486993416829545TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG24300.059.77141628
TATGCCG26500.054.15358448
GTATGCC26750.053.9088647
CACGTGA27100.053.72522431
GTCACGT27300.053.20343429
TCACGTG27750.052.34067530
TCGTATG27500.052.18256445
CACGTCT30250.051.13945414
ACGTGAT28600.051.02984232
ACACGTC30300.050.8243413
ACGTCTG31500.048.77678715
CTCGTAT24900.048.35410344
CGTATGC31400.045.7023446
ATGCCGT32000.045.1740849
GCCGTCT34650.041.012151
CTGAACT38950.039.8059519
TGCCGTC37750.038.478750
TATCGTA5550.037.83815843
GAGCACA41850.037.3825389
GATCCAA40200.036.2179636
Produced by FastQC (version 0.11.2)