FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_5000_200_b2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_5000_200_b2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11716464
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGC146570.12509746968027213TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG31900.042.89992546
CGTATGC35700.038.72537644
GTATGCC37450.037.1026945
CTAATCG40650.034.18106533
CACGTCT42750.034.05750714
TCGTATG40500.034.04928243
AGTCACT41550.033.94608328
CTCGTAT35900.032.95226742
ACACGTC46100.031.9622113
ACGTCTG46350.031.48777415
CACACGT48200.030.93272412
CAGTCAC49750.028.35094827
ACTAATC49700.028.02736732
GTCTGAA54350.027.11053817
AATCGAT50000.026.94934535
CACTAAT54450.026.48225431
ATCGGAA55350.026.1200082
ATGCCGT52700.026.10060147
GTCACTA56450.025.60629
TCACTAA57050.025.45939430
Produced by FastQC (version 0.11.2)