FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_5000_100_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_5000_100_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10082222
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGC119380.11840643858070175TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC26350.039.45086344
TATGCCG27750.036.57854546
GTATGCC31450.031.94051445
TCGTATG32150.031.135943
CTCGTAT31450.028.82365242
CACGTCT36300.028.73162314
CACACGT39050.027.3360512
ACACGTC38950.027.13639513
CGTCTGA39250.026.8396816
ACGTCTG39450.026.70361115
AGTCACC40150.026.5865928
CAGTCAC44850.024.19065327
TCGGAAG43500.023.8990233
ATCGGAA45750.022.7244532
ATGCCGT45650.022.15891647
GTCTGAA47650.021.96133817
TCTCGTA42150.021.25750541
GCACACG57550.019.21748711
AGCACAC58950.019.1767810
GCCGTCT53850.019.04466449
Produced by FastQC (version 0.11.2)