FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_5000_100_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_5000_100_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8852577
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT131370.14839746663598635TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG26150.040.82374248
CGTATGC27850.037.95434646
GTATGCC31200.034.21566847
TATCTCG29600.034.05343241
TCGTATG32350.033.43136645
CACGTCT36300.032.49160414
CTCGTAT32050.031.34161644
ACACGTC39600.030.04927813
CACACGT40100.030.02371412
AGTCACA38800.029.85685728
GTCACAT38450.029.76488529
CGTCTGA40850.029.12961216
ACATTCC39700.028.56322532
CCTTTAT39300.028.31977537
CAGTCAC41800.028.04893527
ACGTCTG42750.027.58936115
TCTCGTA36900.027.0321843
ATCGGAA44650.026.3426152
ATGCCGT40600.026.12168749
TTATCTC39100.026.04811940
Produced by FastQC (version 0.11.2)