FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_5000_050_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_5000_050_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9567523
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT144620.15115720129442073TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG27100.045.9759228
CGTATGC28650.042.02551746
TATGCCG30150.040.28317348
GTCACGT34100.036.8463429
GTATGCC33950.035.46483247
TCGTATG35450.034.55642345
CACGTCT39850.033.98853314
ACGTCCG37450.033.26997832
CGCACAT37950.032.27829437
ACGTCTG43600.031.7073815
ACACGTC43300.031.6040813
GTCCGCA39300.031.52588334
TCACGTC40550.031.33069830
CACACGT44750.030.81482912
CTCGTAT36650.030.17781644
TCCGCAC41550.029.65039435
CCGCACA41550.029.48161936
CGTCTGA46700.029.22789416
ATGCCGT43850.027.85719549
CAGTCAC48250.027.20092827
Produced by FastQC (version 0.11.2)