FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_5000_050_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_5000_050_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7817059
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC110590.1414726433560243TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG20200.046.26064728
CGTATGC21100.043.95795444
TATGCCG20900.043.70957646
GTATGCC24650.038.19500745
CACGTAG24950.038.1546431
TCACGTA26100.036.33940530
TCGTATG26000.035.26949343
GTCACGT26800.035.25987629
CTCGTAT25700.032.82125542
CACGTCT30250.032.39548514
ACGTAGA29300.032.2511532
ACACGTC32150.030.59003313
ACGTCTG33000.029.6958615
CGTAGAG32000.029.42077433
CAGTCAC34550.028.36362827
ATGCCGT32500.028.1086247
CACACGT35500.027.80195612
TAGAGAT35500.026.52047535
CGTCTGA37200.026.34293216
GTAGAGA37450.025.60684834
Produced by FastQC (version 0.11.2)