FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_5000_025_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_5000_025_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9172412
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC163360.1780992829367019TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG28700.046.09606628
CGTATGC29450.044.09241544
TATGCCG30050.043.33039546
GTATGCC33600.039.47982445
GTCACGA33650.039.3154329
TCGTATG34850.037.36053543
CTCGTAT33400.034.47624642
CACGTCT40350.033.3943914
ACACGTC41850.032.4483513
ACGTCTG41700.032.31327415
CACACGT44350.030.8559912
CGTCTGA44900.029.93221316
ATGCCGT44650.029.86741847
CAGTCAC47500.028.8095927
CACGATC48000.028.07200431
GTCTGAA50300.027.34503717
GCCGTCT51950.025.94024749
TCACGAT53100.025.44173430
TCGGAAG53250.025.1099053
TCTCGTA45900.025.08728841
Produced by FastQC (version 0.11.2)