FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_5000_025_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_5000_025_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9570091
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG20800.036.34881646
CACGTCT36600.033.8503414
ACGTCTG37300.032.8397715
CGTATGC21800.032.59328544
AGTCACA38000.032.23465728
ACACGTC39750.030.99193413
GTATGCC25150.029.78271745
CACACTT41250.029.27105131
CGTCTGA44400.027.58836616
CAGTCAC48300.027.02729627
CACACGT46100.027.0266512
TCGTATG24950.026.65428743
TCACACT47500.025.56695730
CTCGTAT25950.025.08723342
GTCACAC48850.024.7169829
GTCTGAA50800.024.38823717
TCGGAAG51850.023.2913783
ACACTTG53450.023.11373732
TTGAATC44750.022.75894736
CTTGAAT53050.022.62853235
Produced by FastQC (version 0.11.2)