FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_2500_200_r.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_2500_200_r.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9687575
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATGC326090.33660642627282883TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG50850.054.24090246
CGTATGC52400.052.43496744
TCGTATG54800.050.2021643
GTATGCC55700.049.45408645
CTCGTAT52400.047.49058542
CACGTCT63750.045.56686814
ACGTCTG65650.043.92823415
AGTCACC66050.043.34427628
ACACGTC67400.043.0475213
ATGCCGT64750.042.8671947
CAGTCAC70100.040.59044627
CACACGT72550.039.89528712
CGTCTGA75000.038.68517316
GCCGTCT70850.038.63340849
GTCTGAA77400.037.7117217
TCTCGTA67900.036.90731441
TCGGAAG79100.036.6406673
ATCGGAA80400.036.1790162
TGCCGTC77000.036.0932448
TCCAGTC80650.035.67128425
Produced by FastQC (version 0.11.2)