FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_2500_200_l.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_2500_200_l.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7969966
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAATATCTCGTATGC607200.7618602137073107TruSeq Adapter, Index 7 (97% over 37bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT365990.4592114947541808No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG147120.1845930083014156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG76950.064.77112646
GTATGCC78700.063.99675445
CACGTCT118400.063.55391714
CTCGTAT79350.063.11886242
ACACGTC119300.063.0451313
ACGTCTG119500.062.88103515
TCGTATG76450.062.76627743
GAATATC112750.062.51743736
AATATCT102300.060.4189737
CTGAACT124600.060.1668119
CGGAATA122400.059.76135334
TATCTCG94250.059.4897539
GAGCACA128500.058.8854689
ATGCCGT82050.058.74020447
GTCACCG124350.058.7116229
GAACTCC127450.058.57422621
CGTATGC83050.058.28468344
GCCGTCT75850.057.2662849
TCCAGTC130950.056.6612225
AGTCACC131850.056.53989828
Produced by FastQC (version 0.11.2)