FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_2500_200_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_2500_200_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14579480
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGC252210.17298970882363432TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC48500.044.38295744
TATGCCG49150.043.7256946
CACCTAG56800.038.756831
GTATGCC56800.038.2670245
TATCTCG52500.038.06691739
TCGTATG58150.037.1378543
CACGTCT63400.036.10285614
ACCTAGC61350.036.0535632
CTCGTAT57500.035.60966542
ACACGTC68000.033.86682513
ACGTCTG68350.033.5393315
CACACGT69400.033.2846112
AGTCACC67850.032.96071628
CAGTCAC71600.031.57658227
CTATCTC66600.030.21729938
ATGCCGT72550.029.81565347
CGTCTGA77800.029.64530416
GCTATCT70950.028.66023837
ATCGGAA80600.028.6202552
TCTCGTA70450.028.56713741
Produced by FastQC (version 0.11.2)