FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_2500_100_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_2500_100_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9386541
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT114510.12199382072693231TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG22900.035.61226748
CGTATGC23050.034.92460346
TATCTCG26100.030.03796441
GTATGCC28050.028.82411447
CACGTCT30600.028.13647814
TCGTATG29200.027.68847745
CTCGTAT28750.027.39142244
AGTCACA31950.026.5091628
GTCACAC33200.025.51188729
ACGTCTG33900.025.5007715
CGTCTGA34600.024.58011216
CACACGT36000.024.20779212
ACACGTC36300.023.71848113
ACTGATA36800.023.01628334
CAGTCAC37750.022.89978627
TCGGAAG38450.022.2133623
TCTCGTA36600.021.80325743
ATGCCGT39250.021.134349
TCACACT41500.020.57818230
GTCTGAA41750.020.53813717
Produced by FastQC (version 0.11.2)