FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_2500_100_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_2500_100_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9626828
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC109050.11327718745987776TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG21500.043.1371228
TATGCCG22850.037.52915646
CGTATGC24200.035.86893544
GTATGCC27850.031.29365545
TCGTATG28100.031.01491743
CACGTCT32550.029.35319714
GTCACGG32800.028.70281429
CTCGTAT28700.028.17135442
ACACGTC34500.027.9985813
ACGTCTG37150.026.18965315
CACACGT38200.025.6531512
GGTAGCA37850.024.9659734
CAGTCAC38800.024.62491427
CGTCTGA41650.023.27601216
ATGCCGT37500.022.96110347
GTAGCAT43650.022.53082735
ACGGTAG42050.022.38876232
GTCTGAA44300.022.12065917
CGGTAGC45850.020.5333133
TCGGAAG46750.020.2919563
Produced by FastQC (version 0.11.2)