FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_2500_050_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_2500_050_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8756601
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC17750.033.71903644
TATGCCG18400.033.2889546
CACGTCT27450.029.19767414
GTATGCC21350.029.01702345
ACGTCTG29700.026.74989315
TCGTATG22450.026.65966643
ACACGTC30450.026.43599313
CACACGT32950.024.32414812
AGTCACC33100.022.94467428
CTCGTAT22500.022.86663842
CAGTCAC34800.021.92438327
CGTCTGA38350.020.62508216
GTCTGAA39000.020.55043617
TCGGAAG40350.019.5191573
ATGCCGT32650.018.97448547
ATCGGAA42400.018.8235262
AGCACAC45400.018.50207510
CTGAACT46200.017.12050819
GCACACG47150.017.07285711
GAGCACA52500.016.733129
Produced by FastQC (version 0.11.2)