FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_2500_050_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_2500_050_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10100546
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT36700.033.9514
CGTATGC20400.031.56977346
TATGCCG21100.030.6886248
AGTCACA40250.030.43347528
CACACGT42750.029.1456712
ACGTCTG42150.029.14494515
ACACGTC43150.028.87534713
GTCACAT42100.028.68076329
CGTCTGA44800.027.88942716
GTATGCC24950.026.93497747
CAGTCAC47400.026.65497427
TCGTATG26150.025.16281345
TCGGAAG51550.024.3768483
ATCGGAA52050.024.0084172
CTCGTAT27950.023.91786844
GTCTGAA53550.023.85518517
CACATGT53350.022.89521631
GTCAGAA54000.022.49010836
GCACACG58450.021.79611811
GATCGGA57950.021.505481
Produced by FastQC (version 0.11.2)