FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_2500_025_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_2500_025_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8023076
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC95300.1187823722472528TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG17950.042.5047428
CGTATGC18750.039.57422344
TATGCCG18900.039.26038746
GTATGCC22200.033.42417545
TATCTCG22100.033.25763339
TCGTATG22500.032.97831743
CGCCAAT24600.031.72657833
CACGTCT26100.030.4392114
CTCGTAT24350.030.18510642
ACGTCTG27200.029.07936315
GCCAATA27350.028.66448634
ACACGTC28300.028.19658313
ATGCCGT27450.026.64921847
CAGTCAC35300.024.3896627
CACGCCA33350.023.612431
CCAATAT33550.023.47193335
CACACGT34650.023.33225812
ACGCCAA34550.023.19748532
GCCGTCT34300.022.34785849
ATATCTC34500.021.91271838
Produced by FastQC (version 0.11.2)