FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_2500_025_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_2500_025_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10420063
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG19150.036.5548846
CGTATGC20450.032.8613844
CACGTCT28250.029.85693414
GTATGCC23750.029.32697945
ACACAGT31050.026.71365732
ACGTCTG31450.026.59646215
TCGTATG24150.026.5223443
AGTCACA30850.026.43292828
ACACGTC31500.026.33215913
CAGTCAC39900.022.9812127
ATGCCGT31350.021.99464647
CACACGT39350.021.4349812
ACAGTGA39850.020.11212334
CTCGTAT22150.020.06807742
GTCACAC40500.019.96197929
GTCGTAT10250.019.46376242
GTCTGAA44150.019.02501717
GCCAGTA30400.018.65721
CACACAG45100.018.54675731
CGTCTGA47350.017.73927316
Produced by FastQC (version 0.11.2)