FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_1250_200_r.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_1250_200_r.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8427422
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGC802900.9527231459395293TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATGTCGTATGC145820.17303037631199672TruSeq Adapter, Index 7 (97% over 36bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT97880.11614465254024303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG123650.064.79500646
CGTATGC125200.064.1575344
GTATGCC126150.064.0909245
TCGTATG124250.063.2674143
CACGTCT143100.063.1981114
ACACGTC145600.062.3052813
ACGTCTG145550.062.1824115
CTCGTAT102300.061.99394242
ATGCCGT131050.060.9229247
GCCGTCT128600.059.2805149
CTGAACT153650.058.5626519
TGCCGTC134800.057.9561248
CGTCTGA157300.057.49300416
TCCAGTC154550.057.40638425
TCGGAAG158450.057.1722263
GAGCACA161250.056.475679
AGTCACC160950.056.36314828
GAACTCC158900.056.18725221
CAGGCGA156150.055.89954434
TCTGAAC161900.055.88109618
Produced by FastQC (version 0.11.2)