FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_1250_200_l.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_1250_200_l.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8799223
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT108270.12304495521934153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT97450.063.06625414
ACACGTC97850.062.8799813
ACGTCTG100150.061.5407515
CTGAACT105100.058.44216519
CATGGCA103550.057.39108334
GAGCACA108450.056.9928559
GAACTCC108450.056.47553621
TCGGAAG116900.052.7288863
TCCAGTC115050.052.71859725
AGTCACC116950.052.49056628
TCTGAAC118350.052.1357918
ACTCCAG118200.051.6985823
GCACACG121000.051.0234711
CGTCTGA121000.050.96533616
AACTCCA120900.050.6019222
GTCACCA122650.048.99560529
CAGTCAC126750.048.8739227
CACACGT127250.048.5174112
GTCTGAA127100.048.46397417
TCACCAT124150.048.31905730
Produced by FastQC (version 0.11.2)