FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_1250_200_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_1250_200_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8378159
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGC917081.0946080159137586TruSeq Adapter, Index 9 (97% over 36bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT244490.2918182860936394No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG101730.12142285673976826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG120300.065.58095646
CGTATGC122600.063.83598744
GTATGCC125500.063.78364645
TATCTCG125700.063.206939
TCGTATG120650.063.01105543
CACGTCT161850.062.99097414
CTCGTAT118100.062.83008242
ACACGTC164300.062.2646913
ACGTCTG163800.062.09152215
ATGCCGT125850.061.5763447
CATTTTA163850.060.62008334
TGCCGTC126800.059.5967948
GCCGTCT121850.059.2602149
CTGAACT176100.057.75428419
AGTCACC174950.057.5737928
TCCAGTC174650.057.5123725
GAACTCC175750.057.3714621
GAGCACA179150.057.2018439
ATTTTAT172900.057.0226835
ACTCCAG177250.056.94518723
Produced by FastQC (version 0.11.2)