FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_1250_100_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_1250_100_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8225902
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG28250.055.7495128
TATGCCG30000.050.86822548
GTCACGA33100.047.68680629
CGTATGC32600.047.5625246
GTATGCC32800.046.84566547
CTCGTAT33700.044.24360344
TCGTATG35300.044.12209345
TATCTCG34300.043.3665741
TCACGAG37750.041.81279830
CACGTCT39150.041.03249714
ACACGTC40650.039.94911213
ACGTCTG40550.039.52953315
CACGAGT40700.038.52416631
ACGAGTG41000.038.24228332
ATGCCGT40300.037.95401449
CAGTCAC44650.036.60517527
GGATATC43550.034.39662638
CACACGT47450.033.92943212
GTCTGAA47950.033.64800617
GATATCT45000.033.13273639
Produced by FastQC (version 0.11.2)