FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_1250_100_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_1250_100_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7122888
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT201910.2834664815732046TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAA29400.058.92720436
TATGCCG30050.055.2113648
ACGTAAT31050.054.89406237
CGTATGC32100.052.1215646
GTATGCC32650.051.13671547
TCGTATG34050.049.34118345
CGTAATC31250.048.94355438
CCGTACG37300.047.2894733
CGTACGT36850.047.01284834
CTCGTAT32800.046.52625344
CACGTCT39500.044.7445414
ACCGTAC40000.044.18492532
ACACGTC40750.043.62966513
ACGTCTG41350.042.8273115
AGTCACC43000.042.0789428
ATGCCGT39350.041.62896749
TAATCTC38350.039.0614740
CAGTCAC46650.038.4114827
GTACGTA46600.037.70272435
GTCTGAA47850.037.2287617
Produced by FastQC (version 0.11.2)