FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_1250_050_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_1250_050_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9553760
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT123290.12904866774966087TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGTA29800.041.6930736
TCCGTAT31450.039.17223437
CGTATGC27250.038.66213646
TATGCCG27700.038.5394948
CGTATCT28100.038.1136539
CCGTATC29800.037.7007238
TATCTCG29300.035.95560541
GTTCCGT35300.035.29612735
CACGTCT38000.034.90695614
AGTTCCG37550.033.27421634
GTATGCC32800.032.33364547
CACACGT40700.032.3336212
ACACGTC41750.031.68793313
CGTCTGA42550.031.17408416
TCGTATG33850.031.01982745
ACGTCTG42700.030.9827715
AGTCACA42000.030.33203928
CTCGTAT34550.029.68221744
CAGTCAC45400.028.6771927
TCGGAAG48100.027.1449033
Produced by FastQC (version 0.11.2)