FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_1250_050_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_1250_050_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8500082
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT101350.11923414385884748TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC21800.041.90407646
TATGCCG21750.041.83973348
CACAGTC25200.036.9432831
GTATGCC24850.036.47922547
CACGTCT27700.033.73599614
TCGTATG27300.033.3334645
ACACGTC28950.032.27953713
ACGTCTG30200.030.59560415
CTCGTAT27850.030.41304244
AGTCACA31150.030.11140828
ACAGTCA31400.029.7602132
CAGTCAC36550.027.38627827
CACACGT34300.027.2446812
ATGCCGT34150.026.9550349
CGTCTGA37850.025.42867516
GTCACAG36400.025.28766329
GTCTGAA39850.023.53752717
TCACAGT39800.023.2153530
GCCGTCT39550.023.18620351
TCGGAAG42050.022.3092233
Produced by FastQC (version 0.11.2)