FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_1250_025_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_1250_025_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9525235
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC22350.037.58421344
TATGCCG22300.037.35555646
GTATGCC25700.032.9574845
TCGTATG26900.031.48659743
CACGTCT29500.030.96539314
ACACGTC32650.028.1923113
ACGTCTG32250.028.10773515
CTCGTAT25800.027.6739342
AGTCACT32400.027.5452328
ACTGACC35750.024.76840232
CACACGT37800.024.0735212
ATGCCGT36400.023.46247947
CAGTCAC42100.022.69520227
CGTCTGA40250.022.26024216
CACTGAC39900.021.92909231
GCCGTCT43350.019.94323349
TCGGAAG44600.019.9352053
TATCGTA4800.019.68715341
GTCTGAA47300.019.68221717
GTCACTG45100.019.55588729
Produced by FastQC (version 0.11.2)