FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_1250_025_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_1250_025_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7952770
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG17500.043.79879832
TATGCCG17450.038.91279646
CACTTAG19850.038.26091431
CGTATGC18250.037.0140844
TCACTTA20950.036.0849330
GTATGCC21200.032.52412445
CACGTCT25050.031.99452614
ACACGTC25800.031.3361513
ACGTCTG25750.030.8529415
TCGTATG21550.029.55946743
TTAGGCA26450.028.9785934
CTCGTAT21100.027.8674142
AGTCACT28000.027.62371828
CAGTCAC35350.024.94933727
ATGCCGT27550.024.77417247
CACACGT33600.023.8533612
CGTCTGA36350.021.95221316
GTCACTT35650.021.69645929
GTCTGAA37550.021.53029617
CTTAGGC37250.020.67057233
Produced by FastQC (version 0.11.2)