FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_0625_200_r.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_0625_200_r.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14922639
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC209810.14059845580932434TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG42950.044.57662646
CGTATGC43300.044.13473544
TCGTATG49100.039.13459443
GTATGCC49600.038.5289145
CACGTCT53450.037.7161414
ACACGTC56050.035.59206813
ACGTCTG59000.034.10894815
CTCGTAT48450.033.88032542
AGTCACC61200.032.99696428
CAGTCAC64250.031.6484627
ATGCCGT62650.030.72752447
CACACGT66350.030.17244512
CTAATCT56800.029.0837637
GTCTGAA73550.027.50402317
GCCGTCT73700.026.2159949
AGCACAC80200.025.74746510
ACTAATC64900.025.39987836
ATCGGAA80950.024.9505352
TCGGAAG81000.024.9348813
CAACTAA81950.024.64210934
Produced by FastQC (version 0.11.2)