FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_0625_200_l.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_0625_200_l.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11190395
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGC314620.2811518270802773TruSeq Adapter, Index 7 (97% over 35bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT181600.1622820284717385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAT43400.058.30508435
TATGCCG41500.058.11070646
GTATGCC42000.057.58481645
TCGTATG42250.056.99478543
CTCGTAT40750.056.60167342
CACGTCT48050.056.30360814
ACACGTC48450.055.6220613
CGATATC43800.055.0557936
ACGTCTG49900.054.0756815
CGTATGC46350.052.70901544
ATGCCGT46100.052.46409247
GCCGTCT48100.049.91901849
CTGAACT55000.049.5068219
CCACGAT53500.047.95128633
ACCACGA53500.047.8856632
TGCCGTC51250.047.192348
CACGATA54500.047.0718734
GAGCACA59700.045.5512929
TATCTCG51850.045.22636439
GAACTCC59650.045.17815421
Produced by FastQC (version 0.11.2)