FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_0625_200_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_0625_200_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13087240
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGC460110.3515714543326171TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG71500.056.9809346
CGTATGC73200.055.13078744
GTATGCC76750.053.40162345
TCGTATG77650.051.70028743
CACGTCT87200.050.3711214
CTCGTAT70900.049.9572642
ACTCAAT86750.049.3413935
ACACGTC91200.048.35392813
ATGCCGT85450.047.88340847
AGTCACC92450.047.2829928
ACGTCTG93000.047.1544315
CCACTCA93150.046.96560333
CAGTCAC94650.046.22094327
CTCAATC83150.044.65884836
GTCTGAA102050.043.1782917
ACCACTC102050.042.7324532
CACTCAA101950.042.63704334
GCCGTCT93700.041.91175549
CACACGT105550.041.87946712
TGCCGTC96400.041.57296848
Produced by FastQC (version 0.11.2)