FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_0625_100_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_0625_100_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8630563
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG17000.042.8215833
AGTCACG18150.040.49402628
ACGTTTC19600.037.4982932
CACGTTT22300.033.11508631
TTCGGAA23000.032.41194536
TTTCGGA23000.032.1074235
GTCACGT25150.029.08416429
CACGTCT28100.027.0277114
ACACGTC30550.025.54756413
TCGGAAT29350.025.28023337
CACACGT30500.025.1304412
CAGTCAC32500.023.79905527
ACGTCTG32850.023.33254215
CGTCTGA32900.023.08432216
GTTTCGG32700.022.6902334
TCGGAAG34100.022.4807593
ATCGGAA36650.020.8214662
GTCTGAA37350.020.80238517
TCACGTT36300.020.34342830
TATGCCG11950.019.03888548
Produced by FastQC (version 0.11.2)