FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_0625_100_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_0625_100_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9520526
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT136690.14357400000798273TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG26050.047.2921228
TATGCCG27000.042.5198148
CGTATGC27100.042.3626946
TCACGTG33700.036.97228230
GCCTTAT33000.036.27230537
GTCACGT34600.035.8082729
GTATGCC32300.035.32596647
TATCTCG32900.033.9364941
TCGTATG34400.032.6603645
CACGTCT42450.031.49501614
ACACGTC42300.031.19316113
ACGTCTG43450.031.0118115
CTCGTAT34400.030.62544644
CACGTGG41450.030.31280331
CCTTATC38100.029.85561238
CACACGT44750.029.64195612
CAGTCAC44000.029.03314427
ACGTGGC44500.027.76328532
ATGCCGT42000.027.0008249
GGCCTTA46000.026.858136
Produced by FastQC (version 0.11.2)