FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_0625_050_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_0625_050_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10113369
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC117240.1159257612374274TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG24300.045.08457646
CGTATGC26800.041.92230644
GTATGCC28400.039.56048245
TCGTATG29200.037.75744243
CACGTCT32100.037.17888314
ACACGTC33800.035.4126613
CTCGTAT27550.034.5557342
ACGTCTG35600.033.7202815
TGTAATC30400.032.811436
AGTCACC40500.030.41827828
CTTGTAA38800.030.21850234
ATGCCGT36900.029.784747
TTGTAAT39550.029.46847335
CAGTCAC43900.027.98269827
TAATCTC36800.027.10587138
GTCTGAA44950.026.70602617
GCCGTCT42800.025.35199749
CACACGT48750.024.69660812
CTGAACT48800.024.59909619
CCTTGTA50300.023.65749233
Produced by FastQC (version 0.11.2)