FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_0625_050_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_0625_050_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9678789
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG19350.029.12300746
CACGTCT34900.028.6806714
CGTATGC18650.028.52640744
TATCTCG20800.028.43784739
ACACGTC37500.026.50548213
ACGTCTG38250.026.44326215
CACACGT38150.026.42084112
AGTCACT36600.026.00976828
GTCACTA36850.025.92841729
TCACTAG37100.025.753730
CGTCTGA38950.025.42890416
CACTAGC38450.024.30333131
CAGTCAC40800.023.58963427
TAGCTTA40800.022.98940734
TCGGAAG43850.022.510923
GTATGCC25150.022.26724845
GTCTGAA47350.021.43515617
ATCGGAA47000.021.3746992
GCACACG51350.020.17436611
CTCGTAT27150.019.46626942
Produced by FastQC (version 0.11.2)