FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_0625_025_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_0625_025_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9901336
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG20800.037.18866746
CACGTCT28650.034.8155614
GTATGCC22600.034.07117545
CGTATGC22400.034.06287844
TATCTCG24150.032.0283739
ACACGTC31250.031.80690813
ACGTCTG31750.031.6367115
TCGTATG24050.031.28917143
CTCGTAT25300.030.29616542
CGATGTA34650.028.78718234
AGTCACC39050.027.3356728
TGTATCT31850.026.04332537
CAGTCAC41550.025.94362427
CACCGAT38500.025.45367231
CACACGT41200.024.89017312
CCGATGT40150.024.494833
GTCTGAA45450.022.40824517
ATGCCGT34050.022.10065747
GTATCTC35700.022.0582938
CGTCTGA46750.021.48578516
Produced by FastQC (version 0.11.2)