FastQCFastQC Report
Wed 18 Jul 2018
HGFFTBGX7_n01_0625_025_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFFTBGX7_n01_0625_025_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11984429
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC184630.15405823673368169TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC34050.041.32324644
TATGCCG34900.040.51789546
GTATGCC38500.036.5467745
TCGTATG40300.035.26166543
CACGTCT45450.032.64985314
CTCGTAT37800.032.2227142
AGTCACA47050.031.31628
ACACGTC48200.030.8599213
GTCACAT46350.030.65632629
ACGTCTG48500.030.59661515
ATGCCGT50600.028.22271347
CAGTCAC58800.026.48672927
ATCACGA59150.026.21177734
CACACGT57500.025.92963612
GCCGTCT56000.025.56384549
CGTCTGA60150.024.72856516
CACGATC49650.024.03762836
GTCTGAA63850.023.89853117
TGCCGTC61000.023.52583148
TCACGAT65400.023.22558635
Produced by FastQC (version 0.11.2)