FastQCFastQC Report
Thu 9 Aug 2018
HGFCJBGX7_n03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFCJBGX7_n03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470897020
Sequences flagged as poor quality0
Sequence length99
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG31559750.6702049208126227No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29302410.6222679005273807No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCA23588430.5009254465020824No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGTG6150780.13061836747236158No Hit
GTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGGTGCAATTG5269090.11189474080766108No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCAGC4962940.10539331932913909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT19442500.073.273591
AGCAGTG20004550.070.989972
CAGTGGT21808050.064.937574
AGTGGTA22355250.063.4319765
GCAGTGG22711100.062.553023
GTGGTAT23843350.059.4816
GGTATCA24072300.058.903288
TGGTATC24779250.057.1427657
GTATCAA30283250.046.7110149
AGAGTAC23285150.034.15973718-19
CATGGGG16871800.034.1436824-25
GCAGAGT23663550.033.6837416-17
TACATGG23777300.033.20298822-23
CAACGCA24532900.032.50007212-13
ACGCAGA24657350.032.3622414-15
ATCAACG25310650.031.55801210-11
AGTACAT25268250.031.39964520-21
CCTCACT6116300.030.53632272-73
CAGAGTA23550100.030.1522618-19
CGCAGAG23881050.029.85963616-17
Produced by FastQC (version 0.11.2)