FastQCFastQC Report
Thu 9 Aug 2018
HGFCJBGX7_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFCJBGX7_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470897020
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCTCAT372021657.9002761580440675No Hit
CCAGGGCA362869587.705922199295294No Hit
GCCACGCT346637057.3612071276220865No Hit
GCCTTGGT301999006.413270570283074No Hit
GCTACCTG301765686.40831577146103No Hit
CTGCAAGC285886386.071101915234036No Hit
AATAATGG269988325.733489670416687No Hit
AAGGGTGA265132455.63037009662962No Hit
AAAGTGCT251223265.334993625570194No Hit
TGCTGTAA250900105.328130978616089No Hit
CGTTAATC234485884.979557526186936No Hit
CTGGACTC226595694.81200093387722No Hit
GTGTCATC224747544.772753499268269No Hit
AAACCTCA222842364.732294971839066No Hit
GGGGGGGG222482854.724660393900985No Hit
TTACTCAG215668224.579944464290728No Hit
TGTAGAAG184310443.914028591644093No Hit
GCCCCGCT6428840.1365232678686308No Hit
CTGGCCTC4986830.10590064893593933No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)