FastQCFastQC Report
Thu 9 Aug 2018
HGFCJBGX7_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFCJBGX7_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470897020
Sequences flagged as poor quality0
Sequence length27
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAATG1150950.017.609191
GACTAAC1150250.017.5769841
CTGCGGA991350.017.525971
CAATGGA1220000.017.4700858
GCGCGAT942750.017.3486481
GGGACCT987950.017.3321671
CGGACTG1156000.017.3287181
ATAACGC1170050.017.2714611
ACTGATG1145950.017.2304441
ATGCGAT1207900.017.1911661
GACTGCG1251500.017.1814791
GACCTGG1212350.017.1644551
TGGGCGT1079600.017.149041
ACGAGGA1099300.017.134121
CTCGAAA1108150.017.0949211
GATGAAA1237300.017.0433161
TGACGGC1122500.016.992421
TTGAACG1112550.016.9187321
ATGGGAG1112650.016.914381
TGACTAG1116900.016.9017451
Produced by FastQC (version 0.11.2)