FastQCFastQC Report
Wed 20 Jul 2022
HGCNNBGXM_n02_12275_totalRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCNNBGXM_n02_12275_totalRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18912060
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6459983.4157992307554017No Hit
CACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGA5230692.7657960053003214No Hit
GAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAA2354971.245221303231906No Hit
CAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTC1552860.8210951107388618No Hit
ACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGG1481280.7832462460461737No Hit
CGGCAAAATAGATCCGTAACTTCGGGATAAGGATTGGCTCTAAGGGTCGG1055880.5583104114517403No Hit
CAAAGATTAAGCCATGCATGTCTAAGTATAAGCAATTTATACAGTGAAAC925540.48939142536561325No Hit
GGGGAAACTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCT751540.3973866411168323No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT717720.37950387213238534No Hit
AAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAG626360.33119607277049673No Hit
AGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTT590320.312139449642186No Hit
AAGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTA558890.2955204245333401No Hit
CGAACGAGACCTTAACCTACTAAATAGTGGTGCTAGCATTTGCTGGTTAT524480.27732568530345186No Hit
TGAAGACTAACTACTGCGAAAGCATTTGCCAAGGACGTTTTCATTAATCA467990.24745585621027008No Hit
AAAACCCATACGCGTAATGAAAGTGAACGTAGGTTGGGGCCTCGCAAGAG464940.24584312867027705No Hit
AGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAAC456450.24135392971469No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGCTGGCGA443890.23471266482868605No Hit
GGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAAT438480.23185205630692796No Hit
AAAAAATCAATGTCTTCGGACTCTTTGATGATTCATAATAACTTTTCGAA429600.22715663973147293No Hit
AAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAA411070.21735865897210563No Hit
CGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGAT405890.21461966596975687No Hit
CAAGAACGAAAGTTAGGGGATCGAAGATGATCAGATACCGTCGTAGTCTT402600.2128800352790759No Hit
AACTACTGCGAAAGCATTTGCCAAGGACGTTTTCATTAATCAAGAACGAA397940.21041599910321773No Hit
CCAAGGTGAACAGCCTCTAGTTGATAGAATAATGTAGATAAGGGAAGTCG375360.19847652767599086No Hit
ACCGAACGTAGAGTTAAGGTGCCGGAATACACGCTCATCAGACACCACAA366320.1936965090000772No Hit
AGAGCTAATACATGCTTAAAATCTCGACCCTTTGGAAGAGATGTATTTAT363100.19199389172834688No Hit
CTTGGATTTATTGAAGACTAACTACTGCGAAAGCATTTGCCAAGGACGTT354710.18755756908554647No Hit
CGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTA343940.18186279019842366No Hit
CGACCCTTTGGAAGAGATGTATTTATTAGATAAAAAATCAATGTCTTCGG341220.1804245544906266No Hit
AACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTC338350.1789070043136496No Hit
CGAGACCTTAACCTACTAAATAGTGGTGCTAGCATTTGCTGGTTATCCAC331890.17549119450763162No Hit
CAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCCTACCATGG330270.17463459824048783No Hit
CAAATGAGAACTTTGAAGACTGAAGTGGGGAAAGGTTCCACGTCAACAGC325550.17213883627695767No Hit
TGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTG320030.16922006381113427No Hit
AACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAATAATGT314570.16633301713298287No Hit
AGCGGCAAAAGCTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGT312710.16534951771515108No Hit
CTTTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTCTG306680.16216107605411573No Hit
CAGATGTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAA299600.1584174331088205No Hit
AATTCTTGGATTTATTGAAGACTAACTACTGCGAAAGCATTTGCCAAGGA296300.15667251478686087No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA290490.1536004010139562No Hit
AACAACTCACCGGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAG284050.15019516647049555No Hit
CCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGCACCTTTGCTGGCTCC281450.1488203823380425No Hit
AGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAA277750.14686395876493624No Hit
ACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTCT275120.1454733117386472No Hit
CGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAG274630.14521421780599258No Hit
CGTTTTCATTAATCAAGAACGAAAGTTAGGGGATCGAAGATGATCAGATA273810.144780632041142No Hit
AATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGATAAGG273150.14443164837675007No Hit
TGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGA272550.14411439050003014No Hit
CTCGACCCTTTGGAAGAGATGTATTTATTAGATAAAAAATCAATGTCTTC270670.14312031581964102No Hit
CTTTCGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGGGA269410.14245407427852916No Hit
AGTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTTAACCTACTAA266440.14088364778876547No Hit
CGGACCAAGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATA265730.1405082259679802No Hit
CGGGTAACGGGGAATAAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACG262690.13890078605926587No Hit
TGTATTTATTAGATAAAAAATCAATGTCTTCGGACTCTTTGATGATTCAT256860.13581809702380387No Hit
AACGAAAGTTAGGGGATCGAAGATGATCAGATACCGTCGTAGTCTTAACC255830.1352734710021013No Hit
AGTTTTCATGCCAGGTCGTACTGATAACCGCAGCAGGTCTCCAAGGTGAA255800.13525760810826531No Hit
AGTCCTTGTTACTTAATTGAACGTGGACATTTGAATGAAGAGCTTTTAGT254350.13449090157285878No Hit
AAGGATTGACAGATTGAGAGCTCTTTCTTGATTTTGTGGGTGGTGGTGCA252560.13354441557397767No Hit
CGTCGGCGCGAGCCCTGGGAGGAGTTATCTTTTCTTCTTAACAGCTTATC249310.13182593540841137No Hit
GGATTCTTCACGGTAACGTAACTGAATGTGGAGACGTCGGCGCGAGCCCT248680.13149281463785542No Hit
CGCTCATCAGACACCACAAAAGGTGTTAGTTCATCTAGACAGCCGGACGG247890.13107509176684085No Hit
TACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCG244930.12950995290835582No Hit
CGAGATTCCCACTGTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGG244790.1294359260704545No Hit
GCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGA237220.12543318919250468No Hit
GAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCTATACTCTAC236610.12511064368450608No Hit
TGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGG233850.12365125745159439No Hit
CCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGGTA233160.12328641089336646No Hit
CAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAGGG227070.12006624344465912No Hit
GCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAATTTATAC224230.11856455616151809No Hit
AGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCA223900.11839006432932213No Hit
CGTTTATTTGATAGTTCCTTTACTACATGGTATAACTGTGGTAATTCTAG222800.11780842488866893No Hit
GCGATAACGAACGAGACCTTAACCTACTAAATAGTGGTGCTAGCATTTGC220210.11643892838749453No Hit
CACAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTG216510.11448250481438829No Hit
TCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTG215000.11368407249130977No Hit
CACGCTCATCAGACACCACAAAAGGTGTTAGTTCATCTAGACAGCCGGAC214830.11359418275957248No Hit
AGATGATCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGG214190.11325577435773787No Hit
GAAAGTGAACGTAGGTTGGGGCCTCGCAAGAGGTGCACAATCGACCGATC212500.1123621646716434No Hit
AGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGT211820.11200260574469412No Hit
CATGCTTAAAATCTCGACCCTTTGGAAGAGATGTATTTATTAGATAAAAA211420.11179110049354751No Hit
CGATCCTAAGAGATGGGGAAGCTCCGTTTCAAAGGCCTGATTTTATGCAG211170.11165890971158088No Hit
ACTTCTTAGAGGGACTATCGGTTTCAAGCCGATGGAAGTTTGAGGCAATA211140.11164304681774487No Hit
CGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCT210360.11123061157800895No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCC207810.10988226560194923No Hit
GAAGTCGGCAAAATAGATCCGTAACTTCGGGATAAGGATTGGCTCTAAGG206640.10926361274234536No Hit
GGTTTATCCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGCACCTTTGC205910.10887761565900277No Hit
CAATGAAGCGGAGCTGGAATTCATTTTCCACGTTCTAGCATTCAAGGTCC205440.1086290969889055No Hit
AGCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGGTTCTGACGTGCAAATC203550.1076297346772377No Hit
CTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTT203240.10746581810759906No Hit
CTAGACAGCCGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTG202080.10685245287927385No Hit
AAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAAAAG201580.10658807131534058No Hit
GATTTGTCTGCTTAATTGCGATAACGAACGAGACCTTAACCTACTAAATA200260.10589010398655674No Hit
AAAATCTCGACCCTTTGGAAGAGATGTATTTATTAGATAAAAAATCAATG197880.10463164774223432No Hit
TGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAAT197660.10451531985410367No Hit
TAAAAAATCAATGTCTTCGGACTCTTTGATGATTCATAATAACTTTTCGA197630.10449945696026769No Hit
CGCGTAATGAAAGTGAACGTAGGTTGGGGCCTCGCAAGAGGTGCACAATC197120.10422978776505575No Hit
CGAATGATTAGAGGTTCCGGGGTCGAAATGACCTTGACCTATTCTCAAAC195230.10323042545338794No Hit
GATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAA194360.10277040153214405No Hit
CCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGG194320.10274925100702938No Hit
CAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAG193920.10253774575588274No Hit
CGGGATAAGGATTGGCTCTAAGGGTCGGGTAGTGAGGGCCTTGGTCAGAC192390.10172873817024693No Hit
GATTCATAATAACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCA192130.10159125975700162No Hit
GCGGAGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGC190810.10089329242821776No Hit
CGAAAGATGGTGAACTATGCCTGAATAGGGTGAAGCCAGAGGAAACTCTG189910.10041740561313786No Hit
GAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGCGGGAGTA189900.1004121179818592No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)