FastQCFastQC Report
Wed 20 Jul 2022
HGCNNBGXM_n02_12274_totalRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCNNBGXM_n02_12274_totalRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36546306
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG457604712.52122991582241No Hit
CGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGAT4120021.1273423913213005No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACG3212790.8791011600461069No Hit
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG3153470.8628696974189403No Hit
CAACGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGAATTGCAA3020570.8265048730232818No Hit
ACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGG3018250.8258700619427857No Hit
GCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATT1743970.47719460347100473No Hit
CGGAGCTGGAATTCATTTTCCACGTTCTAGCATTCAAGGTCCCATTCGGG1638410.4483106993084335No Hit
CTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGAATTGCAATGTCATTTTG1295180.35439423070556025No Hit
CGCTGAACCATAGCAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCTTGG1209130.3308487593794021No Hit
CAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCT1206000.3299923116716639No Hit
CAACTTTCGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGG1183570.3238548924753161No Hit
CGGTTTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCT1163310.31831124053960475No Hit
CAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTC1138520.3115280652441316No Hit
CGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGAATTGCAATGT1108970.3034424327317787No Hit
CGTCGCTGAACCATAGCAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCT1037160.28379338803763093No Hit
GGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAAT999880.27359263067517686No Hit
CGAGGAATTCCTAGTAAGCGCAAGTCATCAGCTTGCGTTGATTACGTCCC973560.2663908084171352No Hit
CATGGTTTCAACGGGTAACGGGGAATAAGGGTTCGATTCCGGAGAGGGAG950920.2601959278729839No Hit
CTGGAATTCATTTTCCACGTTCTAGCATTCAAGGTCCCATTCGGGGCTGA929330.2542883540678503No Hit
TACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCG913040.24983099523109123No Hit
CTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATG876300.2397779956201319No Hit
CGGGGAATAAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA860080.23533979056597404No Hit
AGCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGT842560.2305458724063658No Hit
GCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGA834080.22822552845696634No Hit
GGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCACTT817190.22360399434076866No Hit
TGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGTAATTATTGCTCTTCA802180.2194968761001454No Hit
GCCAGCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTC784520.2146646503753348No Hit
GCCCTGACGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGACCGTA782780.2141885420649627No Hit
CCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGA778640.2130557326368361No Hit
GGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGAATTGCAATGTC771080.21098712411590928No Hit
ATCGGTTTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCC769610.2105848946812846No Hit
AGAGCATTGTAATTATTGCTCTTCAACGAGGAATTCCTAGTAAGCGCAAG745880.20409176237948645No Hit
CGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGGGAATAA715580.1958009107678352No Hit
GGTCTTGGTAATCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGTA705960.19316863378750235No Hit
CAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCG689410.18864013232965324No Hit
CGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGAG663900.18165994669885377No Hit
CTCCGTCGTGCTGGGGATAGAGCATTGTAATTATTGCTCTTCAACGAGGA656230.1795612393766965No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGG638570.1747290136518859No Hit
GGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCA615100.16830702397117783No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG610070.16693068787855056No Hit
AGTAAGCGCAAGTCATCAGCTTGCGTTGATTACGTCCCTGCCCTTTGTAC603770.16520684744444486No Hit
TTTCGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGGGAA591370.16181389166937968No Hit
TGTAATTATTGCTCTTCAACGAGGAATTCCTAGTAAGCGCAAGTCATCAG579910.15867814383210166No Hit
CGAACGAGACCTTAACCTACTAAATAGTGGTGCTAGCATTTGCTGGTTAT577000.15788189372682426No Hit
AAGGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCAC575880.15757543320520545No Hit
ACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATAC574800.15727991770221592No Hit
GGCCTACCATGGTTTCAACGGGTAACGGGGAATAAGGGTTCGATTCCGGA552460.15116712479778394No Hit
GCGCAAGTCATCAGCTTGCGTTGATTACGTCCCTGCCCTTTGTACACACC543870.1488166820471541No Hit
AGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGGGAATAAGGGTTCG535530.146534645662957No Hit
TGGGGATAGAGCATTGTAATTATTGCTCTTCAACGAGGAATTCCTAGTAA534680.14630206401708562No Hit
GTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAG533830.1460694823712142No Hit
CTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGTCGTGCTG532810.14579038439616854No Hit
TGCTCTTCAACGAGGAATTCCTAGTAAGCGCAAGTCATCAGCTTGCGTTG526120.1439598300304277No Hit
TGGTAATCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGTAATTAT524630.14355212808648843No Hit
GGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAAT515470.14104571882039185No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTA514440.14076388459068886No Hit
GCAACGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGAATTGCA513880.14061065432987946No Hit
AGCAACGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGAATTGC502230.13742291765411258No Hit
CTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATG499400.13664855758609365No Hit
CGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGT493310.13498217849979147No Hit
GATGCCCTGACGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGACC493250.1349657609718476No Hit
GGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCC490970.13434189490998077No Hit
AGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAAC487700.1334471396370402No Hit
GGTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAA486050.13299565761858395No Hit
CTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCA476420.1303606443835938No Hit
TGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGG470470.12873257286249395No Hit
CGAAAGGGAATCCGGTTAAGATTCCGGAACCTGGATATGGATTCTTCACG465740.127438324409586No Hit
GGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA463600.12685276591292155No Hit
GCTGGGGATAGAGCATTGTAATTATTGCTCTTCAACGAGGAATTCCTAGT462630.1265873492111624No Hit
GAGGAATTCCTAGTAAGCGCAAGTCATCAGCTTGCGTTGATTACGTCCCT461420.12625626239762783No Hit
CGGGTAACGGGGAATAAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACG460830.1260948233728465No Hit
GTCTGATCAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAA457380.12515081551607432No Hit
CACGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGT456460.12489908008760174No Hit
AACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTC452370.12377995193276169No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGG446260.12210810033714489No Hit
GCAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGC443450.12133921277844059No Hit
CGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGAAAGTGAA440160.12043898499618538No Hit
GGCGGAAAGGCCTTGGGTGCTTGCTGGCGAATTGCAATGTCATTTTGCGT439970.12038699615769648No Hit
AGTGGCCTACCATGGTTTCAACGGGTAACGGGGAATAAGGGTTCGATTCC427940.11709528180495177No Hit
CCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAG421480.1153276612963291No Hit
CGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGG420970.11518811230880625No Hit
CAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATTT416770.11403888535273579No Hit
CCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGTCGTGCTGGGGA414430.1133986017629251No Hit
GCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGTC413530.11315233884376713No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGAGATCGGAAGAGCG397720.10882632023055902No Hit
AAGCGCAAGTCATCAGCTTGCGTTGATTACGTCCCTGCCCTTTGTACACA396730.10855543101948525No Hit
CGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGAGA391470.10711616106973987No Hit
GCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGTCTT390470.10684253560400879No Hit
CCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGG389330.10653060257307537No Hit
AGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTT388320.106254240852687No Hit
CGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGACCGTAAGGTCGG388250.10623508707008583No Hit
GTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTTCTGGGCCGCAC387040.10590400025655122No Hit
GCTGGAATTCATTTTCCACGTTCTAGCATTCAAGGTCCCATTCGGGGCTG386500.10575624250505646No Hit
CAACGGGTAACGGGGAATAAGGGTTCGATTCCGGAGAGGGAGCCTGAGAA384870.10531023299591483No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGG379760.1039120068660291No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGG378610.10359733758043836No Hit
CGCAAGTCATCAGCTTGCGTTGATTACGTCCCTGCCCTTTGTACACACCG377560.10331003084142075No Hit
CGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGTCT375290.10268890103421124No Hit
CGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCTAAGTCAGAAT372800.10200757362454087No Hit
CTAGTAAGCGCAAGTCATCAGCTTGCGTTGATTACGTCCCTGCCCTTTGT372260.1018598158730461No Hit
GGGGAATAAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC370460.10136729003473019No Hit
CATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAAT369000.10096779685476283No Hit
AGGAATTCCTAGTAAGCGCAAGTCATCAGCTTGCGTTGATTACGTCCCTG368560.10084740164984117No Hit
GCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGG367950.1006804901157452No Hit
AACGAACGAGACCTTAACCTACTAAATAGTGGTGCTAGCATTTGCTGGTT365580.10003199776196259No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)