FastQCFastQC Report
Wed 20 Jul 2022
HGCNNBGXM_n02_12273_totalRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCNNBGXM_n02_12273_totalRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25329291
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7045342.7814990952569496No Hit
CGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGAT3303771.304327862947289No Hit
GCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATT2011380.7940924994702774No Hit
ACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGG1923360.7593422176720225No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACG1720810.6793755103528165No Hit
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG1651500.652011933535763No Hit
CAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTC1300140.513295062226574No Hit
GCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGA1245100.49156527910710174No Hit
CGGTTTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCT1219680.4815294672085374No Hit
CGGAGCTGGAATTCATTTTCCACGTTCTAGCATTCAAGGTCCCATTCGGG1083640.4278208971581558No Hit
AGCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGT974010.3845389908466052No Hit
GCCAGCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTC971850.3836862231951143No Hit
CAACGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGAATTGCAA952860.37618897425909No Hit
GGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAAT891000.3517666562400029No Hit
CAACTTTCGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGG840130.33168318844771455No Hit
TGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGG798420.3152160871774895No Hit
CTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATG775910.3063291428094059No Hit
GCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGTCTT765190.3020968885390436No Hit
CTGGAATTCATTTTCCACGTTCTAGCATTCAAGGTCCCATTCGGGGCTGA750110.2961433069721533No Hit
CAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCT694590.27422401993012757No Hit
CAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATTT694090.27402662001080097No Hit
ATCGGTTTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCC672610.2655463194765301No Hit
CGAAGATGATCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTA661390.26111666528684124No Hit
GCCCTGACGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGACCGTA654230.25828989844208433No Hit
AACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTC639480.2524666008219496No Hit
GCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGG602700.23794586275628482No Hit
GCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGTC598530.23629954742910095No Hit
CGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGGGAATAA591740.23361885652464573No Hit
CACGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGT590840.23326353666985783No Hit
GTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTTCTGGGCCGCAC590410.23309377273923693No Hit
GGTCTTGGTAATCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGTA587380.23189752922811777No Hit
GGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCC577550.2280166468141568No Hit
TGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGTAATTATTGCTCTTCA576260.22750735502229413No Hit
CCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGA573840.22655193941275342No Hit
TTTCGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGGGAA571220.22551756383548202No Hit
CGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGT567930.22421867236631296No Hit
CTTTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTCTG562610.22211833722467791No Hit
CGCTGAACCATAGCAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCTTGG560840.22141954151026177No Hit
CTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGTCGTGCTG542210.21406442051615263No Hit
CTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGAATTGCAATGTCATTTTG541720.21387096859521257No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTA537090.21204304534224822No Hit
GGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCACTT530870.2095873903458253No Hit
CATGGTTTCAACGGGTAACGGGGAATAAGGGTTCGATTCCGGAGAGGGAG524210.206958023420395No Hit
CGTCGCTGAACCATAGCAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCT509700.20122947776153702No Hit
CGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGTCT509500.20115051779380638No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG499260.19710776744599762No Hit
CTCCGTCGTGCTGGGGATAGAGCATTGTAATTATTGCTCTTCAACGAGGA487150.19232674139990732No Hit
CGAACGAGACCTTAACCTACTAAATAGTGGTGCTAGCATTTGCTGGTTAT484890.19143449376455107No Hit
CGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGAAAGTGAA476820.18824845906661974No Hit
TGGTAATCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGTAATTAT475620.1877746992602359No Hit
AAGGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCAC459520.18141842185791934No Hit
CAAGAACGAAAGTTAGGGGATCGAAGATGATCAGATACCGTCGTAGTCTT458070.1808459620918722No Hit
ACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGAT450770.17796392326970384No Hit
TACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCG448340.17700455966177656No Hit
CGGACTCTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGC446360.1762228559812432No Hit
CTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGAAAG440790.17402382087994486No Hit
CGGGGAATAAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA436730.17242093353501287No Hit
CTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGTCGTGCTGGGGAT431580.1703877143659489No Hit
CTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTT423510.16720167966801755No Hit
CTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATG411630.1625114575848175No Hit
GGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCA401520.15852003121603364No Hit
GCGGTGATTTCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTC396970.1567236919501616No Hit
CAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCCTACCATGG391830.15469442077948412No Hit
CCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGTCGTGCTGGGGA391150.15442595688919994No Hit
GGCCTACCATGGTTTCAACGGGTAACGGGGAATAAGGGTTCGATTCCGGA390660.15423250496825988No Hit
TTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCC384350.15174131798635818No Hit
CGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTA381290.15053323048007936No Hit
TTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCC379010.14963308684795007No Hit
CAGCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCG377410.14900140710610493No Hit
ACGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGTC374940.14802625150463153No Hit
GCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTTC372250.14696423993865443No Hit
CGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGAATTGCAATGT370020.14608383629845778No Hit
CGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCTAAGTCAGAAT369450.14585880039042545No Hit
CGAGACCTTAACCTACTAAATAGTGGTGCTAGCATTTGCTGGTTATCCAC369370.1458272164033332No Hit
TGGGGATAGAGCATTGTAATTATTGCTCTTCAACGAGGAATTCCTAGTAA368510.14548768854209143No Hit
AGAGCATTGTAATTATTGCTCTTCAACGAGGAATTCCTAGTAAGCGCAAG368100.14532582060824362No Hit
GCGATGGTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTC360720.14241219779898298No Hit
GTCTTGGTAATCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGTAA348450.1375680037787082No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT341430.13479650891136272No Hit
CGAGGAATTCCTAGTAAGCGCAAGTCATCAGCTTGCGTTGATTACGTCCC339710.13411745318887922No Hit
AGACGTTCTGGGCCGCACGCGCGCTACACTGACGGAGCCAGCGAGTCTAA335230.1323487499117129No Hit
GGAACAGTTCATTCGGATAATTGGTTTTTGCGGCTGTCTGATCAGGCATT335100.13229742593268795No Hit
CTGGGCCGCACGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGC333970.13185130211500984No Hit
GCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTTTG333850.13180392613437147No Hit
GTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAG330790.13059583862809265No Hit
AGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGGGAATAAGGGTTCG327200.12917850720732768No Hit
AGTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTTAACCTACTAA323530.12772959179947044No Hit
AGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTT319960.1263201563754785No Hit
GTTCTGGGCCGCACGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTT316800.12507258888533437No Hit
GCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGAAAGTGAACGTAGGT316700.12503310890146904No Hit
AGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCA316100.12479622899827714No Hit
TGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAG314830.12429483320318757No Hit
GCGCAAGTCATCAGCTTGCGTTGATTACGTCCCTGCCCTTTGTACACACC314560.12418823724675121No Hit
TGTAATTATTGCTCTTCAACGAGGAATTCCTAGTAAGCGCAAGTCATCAG311820.12310648568884142No Hit
CCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAG308000.1215983503051862No Hit
CACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGA306060.12083243861819898No Hit
GTCTGATCAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAA305720.12069820667305689No Hit
GGTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAA305070.12044158677793232No Hit
AGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGA300960.11881895944106766No Hit
AACGAACGAGACCTTAACCTACTAAATAGTGGTGCTAGCATTTGCTGGTT298220.11773720788315788No Hit
CGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGACCGTAAGGTCGG297200.11733451204773161No Hit
CGAAAGGGAATCCGGTTAAGATTCCGGAACCTGGATATGGATTCTTCACG297080.11728713606709323No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGCTGGCGA295830.11679363626877673No Hit
GCTGGGGATAGAGCATTGTAATTATTGCTCTTCAACGAGGAATTCCTAGT291560.11510784095772755No Hit
CTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGG291300.11500519299967774No Hit
GCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCACTTG288580.11393133743854102No Hit
CTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCTTGTGGCGT287570.11353258960150128No Hit
CAGGTCTGTGATGCCCTTAGACGTTCTGGGCCGCACGCGCGCTACACTGA284810.11244294204681846No Hit
GGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAA284350.11226133412103798No Hit
TTTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGC283390.11188232627593091No Hit
GGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGAATTGCAATGTC281450.11111641458894368No Hit
ACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATAC281270.1110453506179861No Hit
GCGGAGCTGGAATTCATTTTCCACGTTCTAGCATTCAAGGTCCCATTCGG281250.11103745462121306No Hit
CAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCG281220.11102561062605344No Hit
CGCGGTGATTTCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGT281050.11095849465348241No Hit
CGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAAC280840.11087558668736525No Hit
GGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATA277280.10947009926175982No Hit
CGGTGATTTCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCC274010.10817910378936386No Hit
GATGCCCTGACGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGACC272800.10770139598459348No Hit
GCTGGAATTCATTTTCCACGTTCTAGCATTCAAGGTCCCATTCGGGGCTG272770.10768955198943389No Hit
CGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGG272760.10768560399104735No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA271910.10735002412819214No Hit
ACGAGACCTTAACCTACTAAATAGTGGTGCTAGCATTTGCTGGTTATCCA271130.10704208025404263No Hit
AGTGGCCTACCATGGTTTCAACGGGTAACGGGGAATAAGGGTTCGATTCC269820.10652489246540695No Hit
CCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGG268570.10603139266709045No Hit
CCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGA266640.10526942897848977No Hit
TGCGAAAGCATTTGCCAAGGACGTTTTCATTAATCAAGAACGAAAGTTAG263580.10406134147221097No Hit
GGCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTTT260330.10277824199658805No Hit
GGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAAT257300.10158199848546885No Hit
CAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGCTTGCCTCGGTAAG255070.10070159484527222No Hit
CGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGT253420.10005017511149443No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)