FastQCFastQC Report
Wed 20 Jul 2022
HGCNNBGXM_n02_12270_totalRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCNNBGXM_n02_12270_totalRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30632545
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21542147.0324356007638285No Hit
CGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGAT2846730.9293155367926498No Hit
ACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGG2005080.6545587381002786No Hit
GCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATT1777770.5803533464163686No Hit
CGGTTTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCT1706670.5571427382217181No Hit
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG1669370.54496614629963No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACG1464550.47810261928938647No Hit
GCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGA1374220.44861437402605625No Hit
GCCAGCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTC1118530.36514432607542074No Hit
GCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGTCTT1073730.3505193577614919No Hit
CAACTTTCGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGG1071530.34980116735321864No Hit
CAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTC1066520.34816565192346893No Hit
GTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTTCTGGGCCGCAC1018640.3325352170379575No Hit
ATCGGTTTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCC1015110.3313828478828645No Hit
CGGAGCTGGAATTCATTTTCCACGTTCTAGCATTCAAGGTCCCATTCGGG957070.3124356791118727No Hit
AGCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGT950640.31033660441860117No Hit
CAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCT917680.29957680630192496No Hit
AACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTC866890.28299640137637927No Hit
GGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAAT866180.28276462174461836No Hit
CAACGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGGATTGCAA842750.27511589389650776No Hit
TGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGG831630.27148576783287187No Hit
AAGGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCAC826010.2696511177899192No Hit
CTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATG797790.26043869355288635No Hit
GCCCTGACGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGACCGTA797580.2603701390139148No Hit
CACGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGT768270.250801884074601No Hit
TTTCGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGGGAA758010.2474525051705629No Hit
GCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGG736100.24029998160453206No Hit
GGTCTTGGTAATCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGTA731820.23890277481025493No Hit
CGCTGAACCATAGCAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCTTGG700900.22880893507215938No Hit
GCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGTC690870.2255346397108043No Hit
CGAAGATGATCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTA683930.22326907542288768No Hit
GGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCACTT671450.21919497710686459No Hit
CGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGTCT661230.21585865621024958No Hit
CTGGAATTCATTTTCCACGTTCTAGCATTCAAGGTCCCATTCGGGGCTGA652680.21306750712355113No Hit
GGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCC641310.2093557685135205No Hit
GCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTTTG623950.20368859329187305No Hit
CGTCGCTGAACCATAGCAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCT617310.20152096405963005No Hit
CAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCCTACCATGG607780.19840989379106438No Hit
TGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGTAATTATTGCTCTTCA606120.19786798648300363No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTA602660.19673846884090107No Hit
CGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGGGAATAA597470.19504419237774728No Hit
CAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATTT597040.19490381879794838No Hit
CGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGT593570.19377103665399006No Hit
CCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGA588400.19208328919454784No Hit
CTTTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTCTG579940.18932152062455143No Hit
CATGGTTTCAACGGGTAACGGGGAATAAGGGTTCGATTCCGGAGAGGGAG571250.18648466851187193No Hit
CGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCTAAGTCAGAAT539950.17626677770325644No Hit
CAAGAACGAAAGTTAGGGGATCGAAGATGATCAGATACCGTCGTAGTCTT537790.1755616453024063No Hit
CTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGTCGTGCTG534110.17436030861947643No Hit
GCGGTGATTTCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTC530130.17306103688087296No Hit
TGGTAATCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGTAATTAT528050.17238202049486911No Hit
CGAACGAGACCTTAACCTACTAAATAGTGGTGCTAGCATTTGCTGGTTAT514900.1680892005545083No Hit
GCTGGGGATAGAGCATTGTAATTATTGCTCTTCAACGAGGAATTCCTAGT511550.16699559243281942No Hit
AGAGCATTGTAATTATTGCTCTTCAACGAGGAATTCCTAGTAAGCGCAAG510790.16674749029177954No Hit
TACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCG504230.16460597707438282No Hit
CTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGTCGTGCTGGGGAT496560.16210210415099366No Hit
CGGGGAATAAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA493230.16101502503301635No Hit
GTTCTGGGCCGCACGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTT481350.15713679682834059No Hit
ACGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGTC480380.15682014014832918No Hit
GCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTTC479430.15651001247202934No Hit
GGCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTTT471950.15406816508390014No Hit
AGACGTTCTGGGCCGCACGCGCGCTACACTGACGGAGCCAGCGAGTCTAA470440.1535752253036762No Hit
CTCCGTCGTGCTGGGGATAGAGCATTGTAATTATTGCTCTTCAACGAGGA468410.1529125314269513No Hit
TGGGGATAGAGCATTGTAATTATTGCTCTTCAACGAGGAATTCCTAGTAA464300.15157082116422255No Hit
GGCCTACCATGGTTTCAACGGGTAACGGGGAATAAGGGTTCGATTCCGGA463750.15139127356215426No Hit
CCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGTCGTGCTGGGGA453010.14788519856903826No Hit
CTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCTTGTGGCGT448410.14638352771537594No Hit
GTCTGATCAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAA441370.14408531840890137No Hit
CTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATG438320.14308964534288615No Hit
CTGGGCCGCACGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGC438010.14298844578535672No Hit
TTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCC435570.1421919073325445No Hit
CTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGGATTGCAATGTCATTTTG433060.1413725173667418No Hit
CAGGTCTGTGATGCCCTTAGACGTTCTGGGCCGCACGCGCGCTACACTGA428190.1397827049629732No Hit
GTCTTGGTAATCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGTAA425660.13895678599345893No Hit
GATGCCCTGACGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGACC422290.13785664886805846No Hit
GCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCACTTG420720.1373441220766998No Hit
TTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCC420470.1372625095303051No Hit
ACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGAT415160.13552905904488183No Hit
AACGAACGAGACCTTAACCTACTAAATAGTGGTGCTAGCATTTGCTGGTT413460.13497409372939792No Hit
CGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGAAAGTGAA411820.13443871542504873No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG408880.13347895187944717No Hit
AGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTT404100.13191851999238066No Hit
CGGACTCTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGC397920.12990105784550385No Hit
GCGCAAGTCATCAGCTTGCGTTGATTACGTCCCTGCCCTTTGTACACACC394070.1286442246310256No Hit
GTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAG393360.12841244499926466No Hit
AGTGGCCTACCATGGTTTCAACGGGTAACGGGGAATAAGGGTTCGATTCC389660.12720457931262322No Hit
CCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAG388630.1268683356214771No Hit
TGTAATTATTGCTCTTCAACGAGGAATTCCTAGTAAGCGCAAGTCATCAG387700.12656473694888884No Hit
CTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTT381870.12466153236696462No Hit
CGCGGTGATTTCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGT379920.12402495450508601No Hit
CGAGACCTTAACCTACTAAATAGTGGTGCTAGCATTTGCTGGTTATCCAC379250.12380623288074824No Hit
CAGCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCG377090.1231011004798981No Hit
GGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCA375520.12258857368853943No Hit
CGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTA364930.11913146622326026No Hit
AGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGGGAATAAGGGTTCG355930.11619341455305134No Hit
CAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCG351880.11487129130145733No Hit
AGTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTTAACCTACTAA350650.11446975757319544No Hit
CTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGAAAG350430.1143979385323681No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGG348050.11362098709069063No Hit
TGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAG346910.11324883387913084No Hit
CGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGG339160.11071884494089537No Hit
GGTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAA338450.11048706530913445No Hit
CAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGCTTGCCTCGGTAAG338150.11038913025346081No Hit
TACGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAAC336740.10992883549179476No Hit
CGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGACCGTAAGGTCGG336370.1098080489231306No Hit
CGAGGAATTCCTAGTAAGCGCAAGTCATCAGCTTGCGTTGATTACGTCCC335340.10947180523198448No Hit
GCGGAGCTGGAATTCATTTTCCACGTTCTAGCATTCAAGGTCCCATTCGG329820.10766980020758968No Hit
GGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAA329680.10762409718160863No Hit
AATCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGTAATTATTGCT327610.1069483452974606No Hit
CGGTGATTTCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCC325470.10624974190032203No Hit
CACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCT325060.10611589732423474No Hit
TCGGTTTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCC324090.10579924064422332No Hit
GCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGAAAGTGAACGTAGGT320590.10465666499469763No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG318670.1040298806383864No Hit
ACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATAC316500.10332148373568048No Hit
GGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATA314580.10269469937936923No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGCTGGCGA313660.10239436520863676No Hit
GCAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGC309800.10113426749230271No Hit
TTTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGC306680.10011574291329695No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)