FastQCFastQC Report
Wed 20 Jul 2022
HGCNNBGXM_n02_12269_totalRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCNNBGXM_n02_12269_totalRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29918763
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG408105513.640453651108503No Hit
CGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGAT2346990.7844542235920648No Hit
ACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGG2315670.7739858763545806No Hit
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG1961820.6557156123065649No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACG1446980.48363630541810837No Hit
GCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATT1345470.4497077636531965No Hit
CAACGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGAATTGCAA1241530.41496702253365225No Hit
CAACTTTCGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGG1215410.40623671506739767No Hit
AAGGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCAC1011110.33795180636311734No Hit
TTTCGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGGGAA953500.3186963311283959No Hit
CGGTTTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCT919180.3072252686382789No Hit
GCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGA844750.28234790322046405No Hit
CGGAGCTGGAATTCATTTTCCACGTTCTAGCATTCAAGGTCCCATTCGGG809060.27041893409831147No Hit
GGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAAT785600.2625777008227245No Hit
CGCTGAACCATAGCAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCTTGG770390.25749393449187724No Hit
CTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGAATTGCAATGTCATTTTG763950.2553414390828926No Hit
GCCAGCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTC749240.25042479196081735No Hit
CGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGGGAATAA714760.2389002513238933No Hit
GGTCTTGGTAATCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGTA699580.23382651214557235No Hit
ATCGGTTTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCC682560.22813777427897003No Hit
GGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCACTT673960.2252633238880899No Hit
TGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGTAATTATTGCTCTTCA644260.2153364428870271No Hit
CGTCGCTGAACCATAGCAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCT621670.20778599703470363No Hit
TACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCG620070.20725121556663287No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGG612690.2047845360451567No Hit
AGAGCATTGTAATTATTGCTCTTCAACGAGGAATTCCTAGTAAGCGCAAG604140.20192679757515378No Hit
AGCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGT596010.1992094392405194No Hit
CCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGA591930.19784574649693906No Hit
CGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGAATTGCAATGT575650.19240434505931944No Hit
GCGCAAGTCATCAGCTTGCGTTGATTACGTCCCTGCCCTTTGTACACACC573950.19183613974949432No Hit
GCTGGGGATAGAGCATTGTAATTATTGCTCTTCAACGAGGAATTCCTAGT573660.1917392106084065No Hit
CAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCT573260.19160551524138883No Hit
CGGGGAATAAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA568150.18989755692773796No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG566580.18937280261219358No Hit
AGTGGCCTACCATGGTTTCAACGGGTAACGGGGAATAAGGGTTCGATTCC566050.18919565625089513No Hit
GTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTTCTGGGCCGCAC560790.18743756217461263No Hit
CTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATG540920.18079624481800935No Hit
CAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTC538550.1800040997684296No Hit
CATGGTTTCAACGGGTAACGGGGAATAAGGGTTCGATTCCGGAGAGGGAG528060.17649793876839093No Hit
CTGGAATTCATTTTCCACGTTCTAGCATTCAAGGTCCCATTCGGGGCTGA525360.1755954950410216No Hit
TGGTAATCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGTAATTAT516870.1727578108760713No Hit
GGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCC508570.16998363201045444No Hit
GCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGTCTT507840.16973963796564717No Hit
AACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTC480220.16050797287307633No Hit
GTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAG478330.1598762622639178No Hit
GCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGG474910.15873316687591663No Hit
CTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATG469710.1569951271046868No Hit
GGCCTACCATGGTTTCAACGGGTAACGGGGAATAAGGGTTCGATTCCGGA468060.15644363371573886No Hit
CTCCGTCGTGCTGGGGATAGAGCATTGTAATTATTGCTCTTCAACGAGGA454210.15181443163275166No Hit
GCCCTGACGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGACCGTA450280.15050087465180295No Hit
AGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGGGAATAAGGGTTCG444860.14868930242871337No Hit
GCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGTC440680.1472921858433786No Hit
CGAGGAATTCCTAGTAAGCGCAAGTCATCAGCTTGCGTTGATTACGTCCC439610.14693455073660633No Hit
TGGGGATAGAGCATTGTAATTATTGCTCTTCAACGAGGAATTCCTAGTAA439500.14689778451067645No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGG434940.14537365732667493No Hit
GGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGAATTGCAATGTC432770.144648359960604No Hit
ACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATAC428010.14305738509309357No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTA427320.14282676058498808No Hit
TGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGG422070.14107200889288105No Hit
CGAAGATGATCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTA413650.13825772141715886No Hit
CGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGT408870.13666006178129758No Hit
GCAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGC401580.13422346371740035No Hit
GCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCACTTG398030.13303691733511844No Hit
GTGGCCTACCATGGTTTCAACGGGTAACGGGGAATAAGGGTTCGATTCCG397340.13280629282701292No Hit
CAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCCTACCATGG395620.1322314027488369No Hit
GGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCA392020.13102814444567779No Hit
GTCTTGGTAATCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGTAA386410.12915306692325482No Hit
GCAACGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGAATTGCA380760.12726461986413007No Hit
CTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGTCGTGCTG376620.1258808728154971No Hit
CACGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGT376340.12578728605858472No Hit
AAGCGCAAGTCATCAGCTTGCGTTGATTACGTCCCTGCCCTTTGTACACA368640.12321365024349437No Hit
TGTAATTATTGCTCTTCAACGAGGAATTCCTAGTAAGCGCAAGTCATCAG367950.12298302573538886No Hit
CGAACGAGACCTTAACCTACTAAATAGTGGTGCTAGCATTTGCTGGTTAT367590.12286269990507295No Hit
GTCTGATCAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAA366640.12254517340840596No Hit
CTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGTCGTGCTGGGGAT365060.12201707670868613No Hit
AGTAAGCGCAAGTCATCAGCTTGCGTTGATTACGTCCCTGCCCTTTGTAC358300.11975762500608732No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGG357090.11935319652085882No Hit
CAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCG351390.11744803754085689No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGG350820.11725752164285669No Hit
CCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGTCGTGCTGGGGA350290.11708037528155826No Hit
CGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGTCT344570.11516853153320543No Hit
GGTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAA343210.11471396728534532No Hit
CGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGG324420.10843362741968979No Hit
GCTGAACCATAGCAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCTTGGG324170.10835006781530372No Hit
GCGGAGCTGGAATTCATTTTCCACGTTCTAGCATTCAAGGTCCCATTCGG322010.10762811283340826No Hit
GGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAAT321550.10747436316133792No Hit
GGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA320640.1071702062013727No Hit
TGCTCTTCAACGAGGAATTCCTAGTAAGCGCAAGTCATCAGCTTGCGTTG317550.10613740949116113No Hit
GCGGTGATTTCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTC313270.10470686906407192No Hit
ACGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACG312990.10461328230715956No Hit
GCTGGAATTCATTTTCCACGTTCTAGCATTCAAGGTCCCATTCGGGGCTG312160.10433586442059788No Hit
GAGGAATTCCTAGTAAGCGCAAGTCATCAGCTTGCGTTGATTACGTCCCT309630.10349024122421104No Hit
TTCGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGGGAAT304750.10185915774659535No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)