FastQCFastQC Report
Wed 20 Jul 2022
HGCNNBGXM_n02_12268_totalRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCNNBGXM_n02_12268_totalRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23112037
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5648712.4440554504131335No Hit
CGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGAT2265200.9800953503146433No Hit
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG1649130.7135372792973635No Hit
ACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGG1632280.7062467059913412No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACG1416390.6128365059297889No Hit
GCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGA1361450.5890653428773933No Hit
GCCAGCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTC1201800.519988783333983No Hit
AGCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGT1124500.48654300787074717No Hit
CAACGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGAATTGCAA1074030.4647059019505724No Hit
GCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATT1059160.4582720250923793No Hit
CAACTTTCGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGG1040350.45013340883800074No Hit
CGGTTTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCT968970.41924906921877986No Hit
GCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGTCTT893720.38669027745152884No Hit
GGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAAT845820.3659651462136375No Hit
GGTCTTGGTAATCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGTA841450.36407435657878184No Hit
CACGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGT812440.35152245559316125No Hit
CTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATG811860.3512715041084436No Hit
CGCTGAACCATAGCAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCTTGG728520.3152123718043546No Hit
CAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCT727140.31461528034071595No Hit
CGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGTCT713060.3085232167117074No Hit
GCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGTC693880.30022451071707784No Hit
CGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGT686630.29708761715810683No Hit
GGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCC686020.29682368542417964No Hit
CGGAGCTGGAATTCATTTTCCACGTTCTAGCATTCAAGGTCCCATTCGGG677060.2929469176602651No Hit
CAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTC676630.2927608674215951No Hit
AACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTC675510.2922762714511058No Hit
CGTCGCTGAACCATAGCAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCT667190.288676415670328No Hit
GCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGG656450.2840294864533144No Hit
GGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCACTT655670.28369199997386646No Hit
CTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGTCGTGCTG643690.2785085537895254No Hit
TGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGG622050.2691454673597139No Hit
TTTCGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGGGAA614630.26593501905522215No Hit
GTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTTCTGGGCCGCAC614180.26574031531707915No Hit
TGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGTAATTATTGCTCTTCA613900.2656191663244568No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTA607930.2630360967317593No Hit
GCCCTGACGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGACCGTA602370.26063042387825874No Hit
AAGGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCAC597090.25834590001738056No Hit
ATCGGTTTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCC585950.2535259008109064No Hit
CGAACGAGACCTTAACCTACTAAATAGTGGTGCTAGCATTTGCTGGTTAT584080.25271679861017876No Hit
CGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGGGAATAA575010.2487924365991626No Hit
CCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGTCGTGCTGGGGA566600.2451536400707562No Hit
CGAAGATGATCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTA566220.24498922358076874No Hit
TGGTAATCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGTAATTAT552980.2392606069296272No Hit
CTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGTCGTGCTGGGGAT543210.2350333724370552No Hit
CAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCCTACCATGG539580.23346276228270146No Hit
CTTTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTCTG530200.2294042710298534No Hit
CTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCTTGTGGCGT497760.21536829488460923No Hit
GCGGTGATTTCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTC497440.21522983889304087No Hit
CAAGAACGAAAGTTAGGGGATCGAAGATGATCAGATACCGTCGTAGTCTT497150.21510436315068204No Hit
CATGGTTTCAACGGGTAACGGGGAATAAGGGTTCGATTCCGGAGAGGGAG494700.21404430946523667No Hit
CTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGAATTGCAATGTCATTTTG480670.20797387958491065No Hit
CCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGA479620.20751957086257694No Hit
GTCTTGGTAATCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGTAA454120.19648635903447195No Hit
CTGGAATTCATTTTCCACGTTCTAGCATTCAAGGTCCCATTCGGGGCTGA453650.1962830017968559No Hit
CTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATG449680.19456528215146074No Hit
CAGCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCG449560.1945133611546226No Hit
TACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCG442870.19161876558089622No Hit
CTCCGTCGTGCTGGGGATAGAGCATTGTAATTATTGCTCTTCAACGAGGA434340.1879280480556517No Hit
GCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTTTG434330.1879237213059152No Hit
ACGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGTC432540.1871492331030796No Hit
AGACGTTCTGGGCCGCACGCGCGCTACACTGACGGAGCCAGCGAGTCTAA431430.18666896388232676No Hit
CGAGACCTTAACCTACTAAATAGTGGTGCTAGCATTTGCTGGTTATCCAC421080.1821907779050371No Hit
CGCGGTGATTTCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGT419750.18161532019008103No Hit
CGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCTAAGTCAGAAT405770.1755665240584376No Hit
GTTCTGGGCCGCACGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTT402340.17408244889881408No Hit
AGTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTTAACCTACTAA391850.16954368842521322No Hit
CTGGGCCGCACGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGC385990.16700821307961733No Hit
CGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGG385900.16696927233198874No Hit
CGGGGAATAAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA381770.16518232469080937No Hit
AACGAACGAGACCTTAACCTACTAAATAGTGGTGCTAGCATTTGCTGGTT380640.16469340197058355No Hit
AGAGCATTGTAATTATTGCTCTTCAACGAGGAATTCCTAGTAAGCGCAAG380210.16450735173191353No Hit
CGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGAAAGTGAA373980.1618117866460667No Hit
TGGGGATAGAGCATTGTAATTATTGCTCTTCAACGAGGAATTCCTAGTAA371660.16080798070719599No Hit
CGGTGATTTCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCC370130.16014598799750968No Hit
CGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTA369630.1599296505106841No Hit
GGCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTTT367850.15915948905758504No Hit
GCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCACTTG366490.15857105109341943No Hit
TTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCC364750.15781819663926638No Hit
CACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCT363620.15732927391904056No Hit
CAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCG358470.155100997804737No Hit
CGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGAATTGCAATGT357090.15450390634109837No Hit
CAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATTT354380.15333135716250368No Hit
GATGCCCTGACGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGACC352320.15244004671678227No Hit
GCTGGGGATAGAGCATTGTAATTATTGCTCTTCAACGAGGAATTCCTAGT348930.15097327855610476No Hit
TTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCC348690.1508694365624285No Hit
CAGGTCTGTGATGCCCTTAGACGTTCTGGGCCGCACGCGCGCTACACTGA340320.14724794703296815No Hit
GGCCTACCATGGTTTCAACGGGTAACGGGGAATAAGGGTTCGATTCCGGA339700.1469796885493044No Hit
TGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAG339390.14684555930747253No Hit
CCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAG335470.14514947341075993No Hit
TACGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAAC333770.14441392595555294No Hit
AGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGGGAATAAGGGTTCG328890.1423024720841352No Hit
GGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATA304670.13182308422230374No Hit
CGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCT303850.13146829074390975No Hit
GGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAA296150.1281366934467957No Hit
CGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGACCGTAAGGTCGG295320.12777757321866523No Hit
AATCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGTAATTATTGCT294990.12763479047736034No Hit
CAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGCTTGCCTCGGTAAG292990.126769440530058No Hit
TGTAATTATTGCTCTTCAACGAGGAATTCCTAGTAAGCGCAAGTCATCAG287150.12424261868393513No Hit
GTCTGATCAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAA286470.12394839970185233No Hit
ACGAGACCTTAACCTACTAAATAGTGGTGCTAGCATTTGCTGGTTATCCA284400.1230527625063944No Hit
AGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGA282340.12216145206067298No Hit
GGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGTCGTGCTGGGGATAGA281440.12177204458438692No Hit
CGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGTCG280730.1214648453530946No Hit
AGTGGCCTACCATGGTTTCAACGGGTAACGGGGAATAAGGGTTCGATTCC280420.12133071611126273No Hit
GCTCAAGCGTGTTACCTATACTCTACCGTCAGGGTTGATATGATGCCCTG277140.11991154219768685No Hit
CTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGAAAG275000.11898561775407335No Hit
CGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAAC274240.11865678477409845No Hit
ACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATAC274020.1185615962798952No Hit
GTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAG269930.11679195563766187No Hit
CGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAA269120.11644148890900444No Hit
GGTTGATATGATGCCCTGACGAGTAGGCAGGCGTGGAGGTCAGTGACGAA269080.11642418191005839No Hit
GGTAATCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGTAATTATT268690.11625543867033442No Hit
GGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCA267610.11578814969879114No Hit
GCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGTCGTGCTGGGGATAGAG266980.11551556446539092No Hit
ACGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACG266450.11528624672935579No Hit
CGGACTCTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGC265670.11494876024990787No Hit
GCCGCACGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAG264460.11442522353178994No Hit
GCAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGC262530.11359016083264319No Hit
CGAGGAATTCCTAGTAAGCGCAAGTCATCAGCTTGCGTTGATTACGTCCC259300.11219262066774988No Hit
ACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGAT257000.11119746822835218No Hit
AGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTT256470.11096815049231705No Hit
TTTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGC253380.10963118482373493No Hit
CGAAAGGGAATCCGGTTAAGATTCCGGAACCTGGATATGGATTCTTCACG245230.10610488378847785No Hit
CCGTCGTGCTGGGGATAGAGCATTGTAATTATTGCTCTTCAACGAGGAAT244200.10565922856561713No Hit
CCGAGAGGTCTTGGTAATCTTGTGAAACTCCGTCGTGCTGGGGATAGAGC244080.10560730756877898No Hit
GAGGTCTTGGTAATCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTG243160.1052092465930199No Hit
TGATGCCCTGACGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGAC242500.10492368111041013No Hit
CTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGG241440.10446504563833987No Hit
GGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAAT241070.10430495589808894No Hit
CGCTCAAGCGTGTTACCTATACTCTACCGTCAGGGTTGATATGATGCCCT238870.10335307095605636No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAAC238420.10315836721791333No Hit
GCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTTC237650.10282520748820193No Hit
GACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTG234040.10126325083332118No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG234010.10125027058411164No Hit
GCTGAACCATAGCAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCTTGGG233990.10124161708463862No Hit
CTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTT232160.10044982188285698No Hit
CCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGG231550.10018589014892974No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)